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Development of a direct species-specific PCR assay for differential diagnosis of Leishmania tropica

Authors: 
Milan Jirku
Eva Zemanova
Amer Al-Jawabreh
Gabriele Schfnian
Julius Lukes
Journal Name: 
Diagnostic Microbiology and Infectious Disease
Volume: 
57
Issue: 
1
Pages From: 
75
To: 
79
Date: 
Wednesday, May 17, 2006
Keywords: 
Kinetoplastids, Leishmania tropica, PCR assay, Differential diagnosis
Abstract: 
We have developed a polymerase chain reaction assay for differential diagnosis of Leishmania tropica, based on simple amplification of the target region. The assay detects less than 5 protozoan cells, was tested on human samples and an experimentally infected animal, and is appropriate for clinical laboratories in countries where leishmaniasis is endemic.